Molecular biology

Primers, PCR, cloning, restriction analysis and sequence manipulation.

Reverse complement
Generate the reverse, complement or reverse complement of a sequence.
Built inNo upload
GC content calculator
Measure GC content and base composition of a nucleotide sequence.
Built inNo upload
DNA translation
Translate a nucleotide sequence into protein in any reading frame.
Built inNo upload
Primer3
The reference primer design engine, still the one to beat.
ExternalWhitehead InstituteFree
Primer-BLAST
Primer design with a specificity check against a whole genome.
ExternalNCBIFree
NEBcutter
Restriction mapping and digest planning against a real enzyme catalogue.
ExternalNew England BiolabsFree
Benchling
Cloud plasmid editor and lab notebook, free for academic use.
ExternalBenchlingFree tier
SnapGene Viewer
Free viewer for annotated plasmid maps and sequence files.
ExternalDotmaticsDesktop install
CHOPCHOP
CRISPR guide RNA design with off-target scoring.
ExternalUniversity of BergenFree
Melting temperature calculatorPlanned
Estimate primer Tm, with the choice of model made explicit.
Built inNo upload