Primer-BLAST
Primer design with a specificity check against a whole genome.
Server
Primer-BLAST
NCBI · ncbi.nlm.nih.gov
- Does
- Primer design with a specificity check against a whole genome.
- Access
- Free
Use this when
Any primer that will run against complex template — genomic DNA, cDNA from a whole transcriptome. Slower than Primer3 alone, and worth it.
External tool. We link, we don’t host — nothing you enter on Biology 101 reaches them.
About this tool
Wraps Primer3 and then BLASTs each candidate pair against a chosen database to flag off-target amplification.
Science last reviewed .