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NEBcutter

Restriction mapping and digest planning against a real enzyme catalogue.

Server

NEBcutter

New England Biolabs · nc3.neb.com

Does
Restriction mapping and digest planning against a real enzyme catalogue.
Access
Free

Use this when

Planning a digest you will actually run. It knows which enzymes NEB sells, their buffers and their methylation sensitivity, which generic site-finders do not.

Open NEBcutter

External tool. We link, we don’t host — nothing you enter on Biology 101 reaches them.

About this tool

Maps restriction sites on a sequence, shows predicted gel patterns, and accounts for methylation sensitivity and buffer compatibility.

Science last reviewed .