Primer3
The reference primer design engine, still the one to beat.
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Primer3
Whitehead Institute · primer3.ut.ee
- Does
- The reference primer design engine, still the one to beat.
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- Free
Use this when
Standard PCR primer design. Use NCBI Primer-BLAST when specificity against a whole genome matters more than fine parameter control.
External tool. We link, we don’t host — nothing you enter on Biology 101 reaches them.
About this tool
Designs PCR primers with full control over Tm, GC, product size, self-complementarity and mispriming against a background set.
Science last reviewed .