Serial dilution calculator
Plan a dilution series and get the transfer volume for every step.
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Total volume in each tube after mixing.
fold
| Tube | Concentration | 1 in | Transfer | Diluent |
|---|---|---|---|---|
| Stock | 1 M | — | — | — |
| 1 | 100 mM | 10 | 100 µL | 900 µL |
| 2 | 10 mM | 100 | 100 µL | 900 µL |
| 3 | 1 mM | 1000 | 100 µL | 900 µL |
| 4 | 100 µM | 10000 | 100 µL | 900 µL |
| 5 | 10 µM | 100000 | 100 µL | 900 µL |
| 6 | 1 µM | 1000000 | 100 µL | 900 µL |
Formula
Cₙ = C₀ ÷ fⁿ, transfer = V ÷ f- Conservation of amount of substance on dilution — IUPAC Compendium of Chemical Terminology (the Gold Book), 2019
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About this tool
Generate a full serial dilution plan: the concentration in each tube, how much to carry over, and how much diluent to pre-load. Transfers too small to pipette accurately are flagged rather than left for you to discover at the bench.
Related tools
- Dilution calculator — Work out how much stock and diluent to combine for a target concentration.
- Molarity calculator — Convert between mass, molar concentration and volume for a solution.
Science last reviewed .