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Power and sample size

How many replicates you need — worked out before the experiment, not after.

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Design
Solve for

The smallest difference worth acting on.

%

Per group

64

128 in total

Gives 80.1% power. One fewer drops it to 79.5%.

Formulapower = P(|T′| > t₍₁−α/2₎), T′ noncentral t with δ = d√(n/2)
ModelNoncentral t, two-sided

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Do this before the experiment. Power computed after a non-significant result, from the effect you happened to observe, is circular and tells you nothing.

About this tool

Calculates the sample size required to detect an effect, or the power you have at a given n. Uses the noncentral t distribution rather than a normal approximation, which matters most at the small sample sizes bench experiments actually run at.

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  • t-test calculatorCompare two groups and get the effect size and interval, not just a p-value.
  • One-way ANOVACompare three or more groups, with variance explained and every pair tested.

Science last reviewed .