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EMBOSS Needle

Global pairwise alignment, the reference implementation.

Server

EMBOSS Needle

EMBL-EBI · ebi.ac.uk

Does
Global pairwise alignment, the reference implementation.
Access
Free

Use this when

Two sequences of similar length that you expect to align end to end. Use EMBOSS Water instead when you only expect a shared region.

Open EMBOSS Needle

External tool. We link, we don’t host — nothing you enter on Biology 101 reaches them.

About this tool

Needleman–Wunsch global alignment of two sequences, with the gap penalties and scoring matrix exposed.

Science last reviewed .